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¹ Divisions of Clinical Research and ² Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109
³ Mayo Clinic, Rochester, MN 55905
⁴ Division of Hematology, University of Washington School of Medicine, Seattle, WA 98109
⁵ Ludwig Institute for Cancer Research, 751 24 Uppsala, Sweden

Correspondence to Bruce E. Clurman: bclurman{at}fhcrc.org

The SCF^FBW7 ubiquitin ligase degrades proteins involved in cell division, growth, and differentiation and is commonly mutated in cancers. The Fbw7 locus encodes three protein isoforms that occupy distinct subcellular localizations, suggesting that each has unique functions. We used gene targeting to create isoform-specific Fbw7-null mutations in human cells and found that the nucleoplasmic Fbw7 isoform accounts for almost all Fbw7 activity toward cyclin E, c-Myc, and sterol regulatory element binding protein 1. Cyclin E sensitivity to Fbw7 varies during the cell cycle, and this correlates with changes in cyclin E–cyclin-dependent kinase 2 (CDK2)–specific activity, cyclin E autophosphorylation, and CDK2 inhibitory phosphorylation. These data suggest that oscillations in cyclin E–CDK2-specific activity during the cell cycle regulate the timing of cyclin E degradation. Moreover, they highlight the utility of adeno-associated virus–mediated gene targeting in functional analyses of complex loci.

Abbreviations used in this paper: AAV, adeno-associated virus; CPD, Cdc4 phosphodegron; P382, proline 382; SREBP, sterol regulatory element binding protein; Y15, tyrosine 15.

© 2008 Grim et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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