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Published online July 7, 2008
doi:10.1083/jcb.200804062
The Journal of Cell Biology, Vol. 182, No. 1, 77-88
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Woolner et al.
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Article

 Myosin-10 and actin filaments are essential for mitotic spindle function

Sarah Woolner1, Lori L. O'Brien2, Christiane Wiese2, and William M. Bement1,3

1 Department of Zoology, 2 Department of Biochemistry, and 3 Program in Cellular and Molecular Biology, University of Wisconsin-Madison, Madison, WI 53706

Correspondence to Sarah Woolner: sarah.woolner{at}manchester.ac.uk

Mitotic spindles are microtubule-based structures responsible for chromosome partitioning during cell division. Although the roles of microtubules and microtubule-based motors in mitotic spindles are well established, whether or not actin filaments (F-actin) and F-actin–based motors (myosins) are required components of mitotic spindles has long been controversial. Based on the demonstration that myosin-10 (Myo10) is important for assembly of meiotic spindles, we assessed the role of this unconventional myosin, as well as F-actin, in mitotic spindles. We find that Myo10 localizes to mitotic spindle poles and is essential for proper spindle anchoring, normal spindle length, spindle pole integrity, and progression through metaphase. Furthermore, we show that F-actin localizes to mitotic spindles in dynamic cables that surround the spindle and extend between the spindle and the cortex. Remarkably, although proper anchoring depends on both F-actin and Myo10, the requirement for Myo10 in spindle pole integrity is F-actin independent, whereas F-actin and Myo10 actually play antagonistic roles in maintenance of spindle length.

S. Woolner's present address is Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, UK.

Abbreviations used in this paper: HMM, heavy meromyosin; LatB, latrunculin B; MO, morpholino; Myo10, myosin-10.


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Related In this Issue article

ACTIN AND MYOSIN IN THE MITOTIC SPINDLE
Richard Robinson
J. Cell Biol. 2008 182: 3. [Full Text] [PDF]



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