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Published online July 21, 2008
doi:10.1083/jcb.200802053
The Journal of Cell Biology
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Yamamoto et al.
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ARTICLE

 Spindle checkpoint activation at meiosis I advances anaphase II onset via meiosis-specific APC/C regulation

Ayumu Yamamoto1,2, Kenji Kitamura3, Daisuke Hihara1, Yukinobu Hirose1, Satoshi Katsuyama1, and Yasushi Hiraoka2

1 Department of Chemistry, Shizuoka University, Suruga-ku, Shizuoka 422-8529, Japan
2 Cell Biology Group, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Nishi-ku, Kobe 651-2492, Japan
3 Center for Gene Science, Hiroshima University, Higashi Hiroshima 739-8527, Japan

Correspondence to Ayuma Yamamoto: sayamam{at}ipc.shizuoka.ac.jp

During mitosis, the spindle assembly checkpoint (SAC) inhibits the Cdc20-activated anaphase-promoting complex/cyclosome (APC/CCdc20), which promotes protein degradation, and delays anaphase onset to ensure accurate chromosome segregation. However, the SAC function in meiotic anaphase regulation is poorly understood. Here, we examined the SAC function in fission yeast meiosis. As in mitosis, a SAC factor, Mad2, delayed anaphase onset via Slp1 (fission yeast Cdc20) when chromosomes attach to the spindle improperly. However, when the SAC delayed anaphase I, the interval between meiosis I and II shortened. Furthermore, anaphase onset was advanced and the SAC effect was reduced at meiosis II. The advancement of anaphase onset depended on a meiosis-specific, Cdc20-related factor, Fzr1/Mfr1, which contributed to anaphase cyclin decline and anaphase onset and was inefficiently inhibited by the SAC. Our findings show that impacts of SAC activation are not confined to a single division at meiosis due to meiosis-specific APC/C regulation, which has probably been evolved for execution of two meiotic divisions.

Abbreviations used in this paper: APC/C, anaphase promoting complex/cyclosome; MI, meiosis I; MII, meiosis II; SAC, spindle assembly checkpoint; SPB, spindle pole body.

© 2008 Yamamoto et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).


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