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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200710187
Lee et al.
• Figure S1 Maintenance of tyrosine phosphorylation decreased GTPγS-RhoA binding activity of ROCKI.
• Figure S2 Effect of Y722F mutation on ROCKII-dependent SRF activation in response to serum stimulation.
• Figure S3 Immunofluorescence staining of Y722-phosphorylated ROCKII in HEK293T cells.
• Figure S4 The requirement of Shp2 on RhoAV14-induced cell detachment and MLC phosphorylation in HEK293T cells.
• Figure S5 In vitro Shp2 substrate trapping assay.
• Video 1 Disrupting Y722 phosphorylation of ROCKII decreases cell spreading rate in NIH3T3 fibroblasts.
• Video 2 Effect of Y27632 on cell spreading.
• Video 3 Expression of Y722F mutant of ROCKII delays cell adhesion to FN in D2 cells.
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