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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200802007
Sohn et al.
• Figure S1 The association of antigen-clustered BCR with the raft lipid probe and Lyn kinase differ dramatically.
• Figure S2 The dynamic movement of BCR clusters as they associate with raft lipids and move to the synapse.
• Figure S3 Model for the role of raft lipids in B cell activation.
• Video 1 The dynamic movements of Lyn16-CFP in B cells expressing Igα-YFP and the raft lipid probe Lyn16-CFP as the B cells encounter ICAM-1– and antigen-containing planar lipid bilayers.
• Video 2 The dynamic movements of Lyn16-CFP in B cells expressing Igα-YFP and the raft lipid probe Lyn16-CFP as the B cells encounter ICAM-1– and antigen-containing planar lipid bilayers.
• Video 3 The dynamic movements of Igα-YFP for B cells expressing Igα-YFP and Lyn16-CFP
• Video 4 The dynamics of the association between Igα-YFP and Lyn16-CFP expressed as FRET efficiency for B cells expressing Igα-YFP and Lyn16-CFP as the B cells encounter ICAM-1– and antigen-containing planar lipid bilayers.
• Video 5 Merged images of Igα-YFP and Ea for B cells expressing Igα-YFP and Lyn16-CFP as the B cells encounter ICAM-1– and antigen-containing planar lipid bilayers.
• Video 6 The dynamic movements of LynFL-CFP for B cells expressing Igα-YFP and LynFL-CFP as the B cells encounter ICAM-1– and antigen-containing planar lipid bilayers.
• Video 7 The dynamics of the association between Igα-YFP and LynFL-CFP expressed as FRET efficiency for B cells expressing Igα-YFP and LynFL-CFP as the B cells encounter ICAM-1– and antigen-containing planar lipid bilayers.
• Video 8 Merged images of Igα-YFP and Ea for B cells expressing Igα-YFP and LynFL-CFP as the B cells encounter ICAM-1– and antigen-containing planar lipid bilayers.
• Video 9 The dynamic movement of BCR clusters to the synapse during the contraction phase after antigen binding on the lipid bilayer.
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