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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200805072
Brito et al.
• Figure S1 Indirect immunofluorescence studies reveal that shugoshin (Sgo1) is destroyed as RPE-1 cells slip through mitosis in the presence of nocodazole or vinblastine.
• Video 1 A low-magnification phase-contrast sequence of cultured RPE-1 cells entering and exiting mitosis over a period of 3.5 h
• Video 2 Similar to Video 1 except the culture was treated with 3.2 µM nocodazole and the framing rate was once every 15 min for 50 h
• Video 3 Similar to video 1 except the culture was treated with 250 nM dimethylenastron and the framing rate was once every 10 min
• Video 4 Same as video 1 except the culture was treated with 50 nM nocodazole and the framing rate was once every 10 min.
• Video 5 Near-simultaneous phase-contrast (left) and epifluorescence (right) sequences of a cyclin B/GFP-expressing RPE-1 cell entering and completing mitosis
• Video 6 Near-simultaneous phase-contrast (left) and epifluorescence (right) sequences of a cyclin B/GFP-expressing RPE-1 cell as it enters and exits mitosis in the presence of 50 nM nocodazole
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